Could someone please tell me how to check the specificity of primers in a local computer? I know there are lots of online tools available, i.e. primer-blast. However, the database of my research model is not updated in ncbi or any other database. Now I have a better database available in my local computer and was trying to set up a local primer-blast system. But I cannot find the download link of primer-blast or any replacement softwares to check the specificity of my primers with my local database. Could someone please shed light on this?
Thanks a lot.
Thanks a lot.
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