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  • Strange peak after cDNA enrichment

    Hello,

    Now I am trying to do RNA-Seq for DEG analysis between different tissues of plant root.
    So far, I succeeded to extract total RNA (1-5 ng/ul in 10 ul, RIN ~ 7.0) from cryosectioned samples by using LCM technique.
    I tried SMARTer kit and population Quartz-seq for cDNA synthesis and amplification, but both methods produced a strange peak which is longer than 10kb (High sensitivity DNA kit result attached). I have no idea what this peak is.

    Has anyone seen this kind of huge peak? or Do you have any good ideas to make library using this sample?

    Thanks
    Attached Files

  • #2
    Hi pjtkkw!

    I am preparing single cell cDNA library using Quartz-seq method. Now I have a problem, Aglient 2100 data showed that there was big peak at ~600bp. I used 21x cycles in the PCR. I find that your Aglient 2100 data had the same problem. Did you fix this problem?

    Thank you very much!

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