Hi everyone,
I have been asking around and nobody seems to have a sufficient answer for my question. In ATAC-seq we run a side qPCR reaction in order to determine the appropriate number of PCR cycles to amplify the library without reaching saturation. Why is this only used in ATAC-seq and not other sequencing techniques? Seems to me like it should be used in all sequencing techniques, especially those that are sensitive to PCR duplicates.
Thanks
I have been asking around and nobody seems to have a sufficient answer for my question. In ATAC-seq we run a side qPCR reaction in order to determine the appropriate number of PCR cycles to amplify the library without reaching saturation. Why is this only used in ATAC-seq and not other sequencing techniques? Seems to me like it should be used in all sequencing techniques, especially those that are sensitive to PCR duplicates.
Thanks
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