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Old 10-19-2018, 04:43 PM   #1
gringer
David Eccles (gringer)
 
Location: Wellington, New Zealand

Join Date: May 2011
Posts: 823
Default Flongle flow cells - now shipping

Oxford Nanopore Technologies have started shipping Flongle adapters and flow cells to customers. These are disposable flow cells using fewer pores and a cheaper design ($5000 USD for 48 flow cells). I'd estimate that the best runs with well-prepared flow cells should get about 800 Mb of sequence from a 16h run:

https://twitter.com/leilaluheshi/sta...26561123864576

The lower cost of these flow cells brings in a number of useful applications. In some cases the yield from a flongle flow cell will be more than enough for sequencing experiments (e.g. plasmid sequencing, high-level microbial metagenomic survey). One other example would be to do a quick QC of samples prior to sequencing on a full flow cell: a half-hour sequencing run on a flongle cell should produce sufficient read quantities for digital electrophoresis to determine loading concentrations for the full run.

https://store.nanoporetech.com/flongle.html

These flongle flow cells are compatible with existing MinION sample preparation kits. There's a possibility that ONT might release other flongle-specific kits in the future, but even if not, these flow cells brings the cost per sample down considerably. For example, for a 12-sample rapid barcoding where 50 Mb of sequence per sample might be useful, marginal cost would be ~$115 for sample prep, and $105 for the flow cell, so about $20 per sample.
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