A human protein sequence was compared to a human mRNA sequence using TBLASTN. All amino acids of the protein were matched with 100% identity, but the first (5’) and last (3’) parts of the
mRNA were not included in the alignment, is this because of the UTRs?
When the same mRNA was used in a BLASTN search against the human genome all the mRNA was matched but the alignment was split up in several alignments (HSPs).Why is this?
The human mRNA was compared with BLASTN to the orthologous mRNA from mouse, but BLASTN did not produce any alignment. Why?
I am a beginner in the subject and grateful for answers!
mRNA were not included in the alignment, is this because of the UTRs?
When the same mRNA was used in a BLASTN search against the human genome all the mRNA was matched but the alignment was split up in several alignments (HSPs).Why is this?
The human mRNA was compared with BLASTN to the orthologous mRNA from mouse, but BLASTN did not produce any alignment. Why?
I am a beginner in the subject and grateful for answers!
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