SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Helicos / Direct Genomics



Similar Threads
Thread Thread Starter Forum Replies Last Post
Frozen flow cell Meliss a General 3 09-14-2015 12:49 AM
Maximum clustered flow cell life? pmiguel Illumina/Solexa 5 02-04-2012 09:46 AM
Loading a flow cell with ssDNA (one strand only) Yepler Illumina/Solexa 5 05-23-2011 12:37 PM
Flow cell modifications ? JYC Illumina/Solexa 2 04-08-2011 10:52 AM
sequences on the flow cell suludana Illumina/Solexa 0 07-22-2008 07:51 AM

Reply
 
Thread Tools
Old 01-25-2010, 07:09 PM   #1
Calico
Member
 
Location: Houston

Join Date: Jan 2010
Posts: 13
Default Flow cell/channel terminology

Hello all,

I am a little bit perplexed of the exact mening of the term 'channel' as it is used by Helicos. So, I understand that there machine has two flow cells - in one the synthesis reaction takes place whilst the other is being imaged and this switches back and forth for each added nucleotide. However, each flow cell has 25 independent channels; what does that mean? Does each channel correspond to a picture like this one? Basically, if the HeliScope was a qPCR machine, would it correspond to it being able to run two multiwell plates at the time, with 25 wells per plate?

Edit: Just found a footnote in their product specification sheet stating that for more than 50 samples multiplexing or barcoding is required. This would mean that I am right, right?

Last edited by Calico; 01-25-2010 at 07:14 PM. Reason: Gathered new information.
Calico is offline   Reply With Quote
Old 01-26-2010, 01:00 AM   #2
Xi Wang
Senior Member
 
Location: MDC, Berlin, Germany

Join Date: Oct 2009
Posts: 317
Default

I think you are right that each channel is a independent picture for a scanner to photo. So it is similar with illumina GAs.
__________________
Xi Wang
Xi Wang is offline   Reply With Quote
Old 01-26-2010, 04:56 AM   #3
krobison
Senior Member
 
Location: Boston area

Join Date: Nov 2007
Posts: 747
Default

Looking at the image on the Helicos website may help you -- though I'd suggest any future photographers of these load them up with colored dye to get some higher contrast. Each channel is more than a separate area to photograph, but is an actual separately microfabricated liquid holding region.
krobison is offline   Reply With Quote
Old 01-26-2010, 05:04 AM   #4
Xi Wang
Senior Member
 
Location: MDC, Berlin, Germany

Join Date: Oct 2009
Posts: 317
Default

Sorry I mixed something up. Channel for Helicos may equal to Lane for Illumina.
__________________
Xi Wang
Xi Wang is offline   Reply With Quote
Old 01-26-2010, 06:58 AM   #5
Calico
Member
 
Location: Houston

Join Date: Jan 2010
Posts: 13
Default

Allright, that photo at least clarifies why they call them channels (i.e. they are channels). Though, now I am uncertain what the picture I posted is - a whole flow cell or a segment of a single channel?
Calico is offline   Reply With Quote
Old 01-26-2010, 07:12 AM   #6
Xi Wang
Senior Member
 
Location: MDC, Berlin, Germany

Join Date: Oct 2009
Posts: 317
Default

illumina calls that small square tile. a channel (lane) is made up by several (120 for illunima, 60 rows X 2 cols) tiles.
__________________
Xi Wang
Xi Wang is offline   Reply With Quote
Old 01-26-2010, 07:14 AM   #7
Calico
Member
 
Location: Houston

Join Date: Jan 2010
Posts: 13
Default

And that would be the case for Helicos as well?
Are they imaged sequentially or all simultaneously (in that case, all ties of one channel or of one flow cell at the same time)?
Calico is offline   Reply With Quote
Old 01-26-2010, 07:19 AM   #8
Xi Wang
Senior Member
 
Location: MDC, Berlin, Germany

Join Date: Oct 2009
Posts: 317
Default

sorry, my typo, it's tile, not tie.
they are imaged sequentially. and it is the most time consuming step.
__________________
Xi Wang
Xi Wang is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 08:59 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO