Hi all,
We have some old RNA-seq data sequenced from non strand-specific libraries. Recently we got some new data from the same samples sequenced from strand-specific libraries. We intended to get deeper sequencing but somehow got different preparation.
Can we mix the reads and align them as all non strand-specific reads in STAR? Would it be problematic in the Cuffdiff analysis after alignment?
Thank you!
We have some old RNA-seq data sequenced from non strand-specific libraries. Recently we got some new data from the same samples sequenced from strand-specific libraries. We intended to get deeper sequencing but somehow got different preparation.
Can we mix the reads and align them as all non strand-specific reads in STAR? Would it be problematic in the Cuffdiff analysis after alignment?
Thank you!
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