Hi SOLiD user,
i have to align SOLiD reads to human genome. They are from a paired-end experiment, so I have a csfasta and a quality file for F3 and for F5-P2.
For example I have these files:
solid_data_F3.csfasta
solid_data_F5-P2.csfasta
solid_data_F3_QV.qual
solid_data_F5-P2_QV.qual
To obtain a fastq file, is correct the following command?
> solid2fastq.pl solid_data_ output
In this way, i obtain only one file to align to genome. Is it correct?
thanx to everybody
ME
i have to align SOLiD reads to human genome. They are from a paired-end experiment, so I have a csfasta and a quality file for F3 and for F5-P2.
For example I have these files:
solid_data_F3.csfasta
solid_data_F5-P2.csfasta
solid_data_F3_QV.qual
solid_data_F5-P2_QV.qual
To obtain a fastq file, is correct the following command?
> solid2fastq.pl solid_data_ output
In this way, i obtain only one file to align to genome. Is it correct?
thanx to everybody
ME
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