Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Control DNA sequencing primer

    I have looked over the excellent post above containing the sequences for all of the Solexa adapter/primer sequences (thanks ECO). However it did not specifically mention the sequencing primer for the phiX174 control DNA. Is the sequencing primer for the phiX control the same as the standard genomic sequencing primer?

    Thanks

  • #2
    I'm not the person to ask...all the information I have at this time is in that Sticky post. I'm sure someone will chime in. I would expect them to be the same, but you never know.

    Comment


    • #3
      Yes, it is the same primer - also the same primer used for smRNA sequencing, would be helpful if they let us know if it were the same for all kits

      Comment


      • #4
        No, for DGE kits the primer used is a different one

        Comment


        • #5
          Interesting.....I looked at the website that shows the primer sequences, they appear to be different for all the kits - but I have used the same primer for PhiX, smRNA, and genomic sequencing. I wonder if the ligated pieces are the same and they just choose different parts to design primers for different kits?

          Comment

          Latest Articles

          Collapse

          • seqadmin
            Recent Advances in Sequencing Analysis Tools
            by seqadmin


            The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
            Today, 07:48 AM
          • seqadmin
            Essential Discoveries and Tools in Epitranscriptomics
            by seqadmin




            The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
            04-22-2024, 07:01 AM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by seqadmin, Today, 07:17 AM
          0 responses
          11 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 05-02-2024, 08:06 AM
          0 responses
          19 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 04-30-2024, 12:17 PM
          0 responses
          20 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 04-29-2024, 10:49 AM
          0 responses
          28 views
          0 likes
          Last Post seqadmin  
          Working...
          X