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Globin & rRNA removal from blood RNA epibio Vendor Forum 0 02-18-2013 07:20 AM

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Old 08-22-2016, 01:13 AM   #1
kaarelkrj
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Location: Sweden/Estonia, EU

Join Date: Aug 2009
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Default Globin reduction for RNA-seq by two oligos

Dear community,
we published a detailed globin locking (GlobinLock) methodology to overcome blood sample analysis limitation caused by abundant globin mRNA from erythrocytes. The locking DNA molecules bind specifically at globin RNA poly-A site that is needed for further analysis. Therefore the globin RNAs are “locked” prior downstream manipulations. The GlobinLock is universal for multiple RNA-seq methods like SMART-Seq, G&T-seq, CEL-seq and STRT where anchored oligo-T primer is applied.

In addition to human, GlobinLock is effective for mouse, rat, cow, dog and zebrafish

Access to open paper: http://www.nature.com/articles/srep31584
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File Type: jpg GlobinLock.jpg (86.1 KB, 10 views)

Last edited by kaarelkrj; 08-22-2016 at 02:38 AM.
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