Hello,
Does anyone know of a standalone kit or protocol which could deplete mammalian rRNA and bacterial rRNA to be left with bacterial RNA we want for sequencing? The best way I know how seems to be a convoluted mess of ribozero, microbe enrich, and truseq by illumina while skipping the poly-A selection step. Surely there has to be a more straightforward way to achieve desired RNAseq experiments for clinical bacterial samples?
Does anyone know of a standalone kit or protocol which could deplete mammalian rRNA and bacterial rRNA to be left with bacterial RNA we want for sequencing? The best way I know how seems to be a convoluted mess of ribozero, microbe enrich, and truseq by illumina while skipping the poly-A selection step. Surely there has to be a more straightforward way to achieve desired RNAseq experiments for clinical bacterial samples?
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