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Old 05-21-2019, 09:46 PM   #1
lijing2019
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Unhappy discrepancy between qubit and bioanalyzer results

When I do the QC for my bead cleaned-up pre-amplified cDNA (Smartseq2) using AATI fragment analyzer and bioanalyzer, the concentration was very low (0.01ng/uL) and almost no peaks. But when I measured the same samples using Qubit, I got about 0.1ng/uL. Why are these methods giving different concentrations? Thanks!
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Old 05-22-2019, 09:19 AM   #2
cmbetts
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The FA/BA is probably a more accurate measurement (although I trust qubit more for concentrations >1ng/ul). It's easy to get background readings of 0.1-0.3ng/ul by qubit. Did you run a negative control for comparison?
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Old 05-22-2019, 08:03 PM   #3
lijing2019
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Quote:
Originally Posted by cmbetts View Post
The FA/BA is probably a more accurate measurement (although I trust qubit more for concentrations >1ng/ul). It's easy to get background readings of 0.1-0.3ng/ul by qubit. Did you run a negative control for comparison?
OK, I see. I will include a negative control to see how it goes. Thank you for your reply.
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