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Old 07-09-2019, 05:35 AM   #1
baru
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Default Agilent SS All Exon V7 kit

Anyone here using Agilent SS All Exon V7 kit? I'm continuously getting low yield around 2-3 nM with 100ng input. Any suggestions?
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Old 07-09-2019, 11:43 AM   #2
itstrieu
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Which library and capture kit are you using? I tested the Exon V7 probes with SureSelectXT and SureSelectXT HS a while back. XT gave >50 nM while XT HS gave around 10 nM. Started with 200 ng as the starting concentration.
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Old 07-10-2019, 10:55 AM   #3
baru
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Hey thanks! I am using SS XT Low Input kit with dual index. While I did the test run I got really good yield (around 10-12nM) with 100ng input. I am starting to think it could be reagent issue
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Old 07-10-2019, 11:41 AM   #4
itstrieu
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SS XT Low Input has a similar workflow to HS. After library prep, 1000 ng was inputted into hybridization. Other places where I see can affect yield is lost of the capture beads during the washing step.

Are these the same samples as your test run? They could be DNA of poorer quality.
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