Hi everybody,
I would like to run whole genome sequencing in a high throughput manner.
Libraries are prepared using Illuminas TruSeq PCR-free protocol and chemistry.
The time and cost consuming step is the final quantification with qPCR. I used the KAPA lib quant kit.
I was wondering if the is another method to quantify the final libraries. Just picoGreen seems not to be the best option to get reliable results because we measure also fragments with one or do adapter.
The whole genome libs should be run on a HiSeqX.
Thanks,
Elisabeth
I would like to run whole genome sequencing in a high throughput manner.
Libraries are prepared using Illuminas TruSeq PCR-free protocol and chemistry.
The time and cost consuming step is the final quantification with qPCR. I used the KAPA lib quant kit.
I was wondering if the is another method to quantify the final libraries. Just picoGreen seems not to be the best option to get reliable results because we measure also fragments with one or do adapter.
The whole genome libs should be run on a HiSeqX.
Thanks,
Elisabeth
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