Hey everyone,
Another kind of barcoding question for pacbio (see fellow poster chammer's question on http://seqanswers.com/forums/showthread.php?t=44489 )
But we've also been thinking about multiplexing samples (>100-300) for Pacbio sequencing using barcoded SMRTbell adapters as we have limited input amounts of our DNA libraries. Also, these barcodes are (more or less) optimized for Pacbio, hence we'd favour these.
Could anyone share their experiences using this approach? Mainly in terms of advantages vs. disadvantages/limitations, etc.
For other approaches such as barcoded PCR primers you could theoretically multiplex 384 samples, whereas it seems that for barcoded adapters it seems to be only possible for 12 samples according to the following link;
Could anyone clarify this for me? It'd be much appreciated!
Thanks!
m
Another kind of barcoding question for pacbio (see fellow poster chammer's question on http://seqanswers.com/forums/showthread.php?t=44489 )
But we've also been thinking about multiplexing samples (>100-300) for Pacbio sequencing using barcoded SMRTbell adapters as we have limited input amounts of our DNA libraries. Also, these barcodes are (more or less) optimized for Pacbio, hence we'd favour these.
Could anyone share their experiences using this approach? Mainly in terms of advantages vs. disadvantages/limitations, etc.
For other approaches such as barcoded PCR primers you could theoretically multiplex 384 samples, whereas it seems that for barcoded adapters it seems to be only possible for 12 samples according to the following link;
Could anyone clarify this for me? It'd be much appreciated!
Thanks!
m
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