Hi,
I have a file with mixed paired end reads (50bp and 70bp). My insert size is 400bp. Did anyone try running tophat with mixed paired end reads? If so, what would be the tophat -r option (will it be 300bp or 260bp or an average 280bp)?
Thank you in advance.
Nirmala
I have a file with mixed paired end reads (50bp and 70bp). My insert size is 400bp. Did anyone try running tophat with mixed paired end reads? If so, what would be the tophat -r option (will it be 300bp or 260bp or an average 280bp)?
Thank you in advance.
Nirmala
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