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Old 08-30-2017, 09:10 AM   #1
bohawk
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Location: Iowa

Join Date: Aug 2017
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Default Program for clustering rna seq reads using protein database

I have an RNA Seq data that I have used to assemble a de novo transcriptome, however, I am having trouble clustering it down into a final set of putative transcripts. I have used the Corset pipeline to cluster it down to 160,000 "genes" right now but beyond that I am stuck.

I have two unpublished protein databases for close relatives of my model and I was wondering if anyone had any suggestions for programs to map my putative transcripts to and cluster them even more?
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clustering, de novo, mapping reads, proteome, transcriptome

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