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Old 03-27-2019, 01:27 AM   #1
Junior Member
Location: Taiwan

Join Date: Mar 2019
Posts: 1
Default please help me to figure out what's wrong in my Ribomus library preparation

Hi all,

I used TruSeq stranded total RNA sample preparation guide to remove ribosomal RNA. However, my final library had a high peak in bio-analyzer data (as attached file).

My condition list below:
RIN of RNA: 10
input RNA: 1 ug
digest time: 8 mins as protocol
another condition was by the protocol.

Could anyone help me to figure out which step I should modify?

Many thanks.

Aero Ma
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