Does anyone know what the exact primer sequences are for the cancer panels? I really need to know so I can remove them during the alignment step. Otherwise it causes certain indels to be missed.
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Hi there,
After logging in to the AmpliSeq Designer website, navigate to the Ion Panels page. From there you can choose to 'Download panel files" for the panel you need. The pop-up window describes the contents of all the files in the zip archive, including the *.primerDataSheet.csv file to which you are referring.
I would also recommend follow up with your local Field Bioinformatics Scientist if you have any questions about analysis of the data with which they may be able to assist.
Originally posted by RockChalkJayhawk View PostDoes anyone know what the exact primer sequences are for the cancer panels? I really need to know so I can remove them during the alignment step. Otherwise it causes certain indels to be missed.
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Originally posted by snetmcom View Postaren't the majority of the primer sequences removed in the library protocol?
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