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Old 06-12-2010, 11:37 AM   #1
polsum
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Default Bowtie nor mapping to RNA database

Does bowtie recognize only T and not U? When I used RNA database none of my reads mapped to it, but when changed U to T then they mapped. am I missing something? thanks.
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Old 06-12-2010, 05:26 PM   #2
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Quote:
Originally Posted by polsum View Post
Does bowtie recognize only T and not U? When I used RNA database none of my reads mapped to it, but when changed U to T then they mapped. am I missing something? thanks.
You may have missed reading the manual (highly advised).

Quote:
Alignments involving one or more ambiguous reference characters (N, -, R, Y, etc.) are considered invalid by Bowtie. This is true only for ambiguous characters in the reference; alignments involving ambiguous characters in the read are legal, subject to the alignment policy. Ambiguous characters in the read mismatch all other characters. Alignments that "fall off" the reference sequence are not considered valid.
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