SEQanswers

Go Back   SEQanswers > Bioinformatics > Bioinformatics



Similar Threads
Thread Thread Starter Forum Replies Last Post
Gene Ontology Program syintel87 Bioinformatics 1 05-23-2014 10:22 AM
Gene Ontology Graphs Quat Bioinformatics 3 09-06-2012 08:35 AM
gene ontology ssing Bioinformatics 1 02-22-2012 05:24 AM
window cutoff for nearest gene (gene ontology) repinementer Bioinformatics 1 11-22-2010 05:40 PM

Reply
 
Thread Tools
Old 05-21-2015, 05:59 PM   #1
michael-steffen
Member
 
Location: Tulsa, OK

Join Date: Dec 2012
Posts: 18
Default Gene ontology post IDing using Blast on Geneious

I have a few thousand sequences which I have annotated from blasting to NCBI. I would like to add GO terms to them. I looked into using Blast2go, however, it seems like I have to re-blast sequences and then I can apply go terms. The whole reason I used geneious blast to ncbi was because Blast2go was so slow. Is there a way to by pass the initial blasting again or some other program which is faster.

Thanks,
Mike
michael-steffen is offline   Reply With Quote
Old 05-26-2015, 06:52 AM   #2
sgoetz
Member
 
Location: Spain

Join Date: May 2010
Posts: 11
Default

Hi Mike.
In which format did you save and with which parameters did you run your blast searches? In Blast2GO you can import Blast results as XML. This is possible with the Blast2GO PRO Plugin for Geneious (get a trial from here: https://www.blast2go.com/b2g-plugins/geneious-plugin) as well as the free Blast2GO Basic. In case you do not have your results in XML you would need to redo them. Just for your information, with the PRO (plugin and normal) version you can use Blast2GO's CloudBlast to run NCBI blast+ searches faster than via the NCBI webservice or locally.
Hope this helps.
Best, Stefan
sgoetz is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 06:25 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO