Go Back   SEQanswers > Sequencing Technologies/Companies > Oxford Nanopore

Similar Threads
Thread Thread Starter Forum Replies Last Post
Library size selection LacquerHead General 9 07-26-2016 10:33 AM
Always get the same insert size after PCR despite initial size selection on gDNA melop Sample Prep / Library Generation 10 07-13-2016 11:41 PM
Help with size selection ptery General 2 07-17-2015 12:31 PM
size selection cmccabe Sample Prep / Library Generation 2 11-19-2014 10:59 AM
PE (in gel) Size-Selection puggie Illumina/Solexa 3 11-28-2011 04:24 AM

Thread Tools
Old 07-12-2019, 04:27 AM   #1
Junior Member
Location: Germany

Join Date: Oct 2018
Posts: 4
Default Circulomics Size Selection

Hi Community,

I tried today for the first time the Circulomics Size Selection Kit with DNA for a library for MinION.
I started with 5 g DNA with known size (above 40kb, but with fragments lower 10kb)), but after the 30 minutes centrifuging I didn't see a pellet. I processed the sample after that as it was written in the protocol, but the Qubit said, I nearly lost all my sample.

Did I miss something? What can I improve to keep my DNA?
Do you have any other tipps I could follow?

Thank you in advance!
Femta is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 09:18 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO