Hi,
our lab is currently sequencing human exomes on Ion Proton Sequencers.
Now, we plan to give the NextSeq500 a try (first steps into the Illumina world).
We plan on using the Rapid Capture Exome Kit for whole Exomes and the TrueSight One Kits for clinical exomes.
The aim is to cover >90% of Bases >20x.
How many samples do you pool on a High-Output run (300 cycles) to get reliable exome data? Is 12 a realistic number for whole exomes and 36 for clinical exomes (TrueSight One)?
best,
Sören
our lab is currently sequencing human exomes on Ion Proton Sequencers.
Now, we plan to give the NextSeq500 a try (first steps into the Illumina world).
We plan on using the Rapid Capture Exome Kit for whole Exomes and the TrueSight One Kits for clinical exomes.
The aim is to cover >90% of Bases >20x.
How many samples do you pool on a High-Output run (300 cycles) to get reliable exome data? Is 12 a realistic number for whole exomes and 36 for clinical exomes (TrueSight One)?
best,
Sören
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