Hello everyone!
I am trying to prepare a library composed of amplicons of around 100 bp. After the PCR I checked the presence of amplification and then I did a cleaning step using AMPure XP beads. After this cleaning step, I lost practically all my amplicons. I tried to increase the concentration of beads (from 1.5X to 2.5X) but the result is exactly the same... Anybody know what is happening?
Thanks!
Inaki
I am trying to prepare a library composed of amplicons of around 100 bp. After the PCR I checked the presence of amplification and then I did a cleaning step using AMPure XP beads. After this cleaning step, I lost practically all my amplicons. I tried to increase the concentration of beads (from 1.5X to 2.5X) but the result is exactly the same... Anybody know what is happening?
Thanks!
Inaki
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