Hi
I am a new SOLiD user, and completed our first RNA-Seq experiment. I have a question regd the secondary analysis report. For the secondary analysis we uploaded whole human genome as reference and the % matched summary report for our 2 samples were around 25%. But according to ABI the run is considered successful only if the % match is above 30%. Can anyone comment on what the problem might be of low % match.
Thanks
I am a new SOLiD user, and completed our first RNA-Seq experiment. I have a question regd the secondary analysis report. For the secondary analysis we uploaded whole human genome as reference and the % matched summary report for our 2 samples were around 25%. But according to ABI the run is considered successful only if the % match is above 30%. Can anyone comment on what the problem might be of low % match.
Thanks
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