Hey all,
I am attempting to pool RNA from single cells that have been FACS sorted in an attempt to increase my RNA concentration before cDNA amplification and library prep. The reason I have to pool instead of sorting more cells/well is that I have to determine if the RNA in each well contains viral RNA or not. I will then pool cells with or without viral RNA. The issue is that if I start pooling RNA samples in lysis buffer my lysis buffer concentration will quickly surpass the maximum amount required for Clontech's first-strand cDNA step. What would be an effective way to concentrate the RNA without losing it or degrading it?
Cheers!
I am attempting to pool RNA from single cells that have been FACS sorted in an attempt to increase my RNA concentration before cDNA amplification and library prep. The reason I have to pool instead of sorting more cells/well is that I have to determine if the RNA in each well contains viral RNA or not. I will then pool cells with or without viral RNA. The issue is that if I start pooling RNA samples in lysis buffer my lysis buffer concentration will quickly surpass the maximum amount required for Clontech's first-strand cDNA step. What would be an effective way to concentrate the RNA without losing it or degrading it?
Cheers!
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