Hi Folks,
as an academic sequencing service provider we are obligated to store the sequencing "rawdata".
We are currently defining "rawdata" as the "standard" rundata plus CIFs (for HiSeq devices).
Amount of data is roughly 3-4TB per run. 80%-85% being CIFs.
In the past this enabled us to run OLB on lanes/runs to "optimize" basecalling in some cases.
OLB development has been dropped some time ago, RTA was developed continously.
So my questions are:
* What files/data are you (permanently) storing of HiSeq run? And why?
* Are you storing the data for (potential) re-analysis (OLB?) or just for documentation purposes?
* Anyone with experience in running OLB 1.9.x on HiSeq data created with current machine software?
I am just curious :-)
best,
Sven
as an academic sequencing service provider we are obligated to store the sequencing "rawdata".
We are currently defining "rawdata" as the "standard" rundata plus CIFs (for HiSeq devices).
Amount of data is roughly 3-4TB per run. 80%-85% being CIFs.
In the past this enabled us to run OLB on lanes/runs to "optimize" basecalling in some cases.
OLB development has been dropped some time ago, RTA was developed continously.
So my questions are:
* What files/data are you (permanently) storing of HiSeq run? And why?
* Are you storing the data for (potential) re-analysis (OLB?) or just for documentation purposes?
* Anyone with experience in running OLB 1.9.x on HiSeq data created with current machine software?
I am just curious :-)
best,
Sven
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