Originally posted by Simone78
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I tried to produce the Tnp once again with the C3013 lately. But the strain seemed to reach plateau phase after the A600=1.3. No matter there is IPTG induction or not, same thing happened. However, according to your protocol, the A600 should reach 2.1 after IPTG induction for around 4h. The protein solution is still being dialyzed, I will test if there is any activity after that in any case. But the strain seems weird on our hands. Maybe you can give me some advices. Thanks!
Cheers,
Gary
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