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I've heard from some people that SPRI is not a great way to concentrate DNA products. I usually use 0.6x SPRI on 40-50 uL of PCR product and elute into 30-40uL of water. I am wondering if I could concentrate the product further by just using lower volume of water (or TE, or any elution buffer), like maybe 10uL. Anyone here has any experience doing that? A lot of protocols seem to suggest putting the elutted product on a column to concentrate, which I think kinda defeats the purpose of using SPRI...
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I usually take a 50 ul PCR reaction and elute into 22.5 ul. Ten ul seems extreme - you may have problems pipetting up the liquid without beads. -
I elute 50 uL PCR products in 20 uL pretty regularly without any issues or loss in yield. When going below 20 uL, I start to see a drop in total recovery.Josh KinmanComment
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