Hi all,
I'm new in the ChIP-seq world. I have a good antibody that I know works well in ChIP and I also know some good target regions that I can use to validate the ChIP. After performing and validating a ChIP I used the ChIP'd material to construct a library to use for Solexa sequencing. To test if the library construction went fine I tried to perform qPCR validation using the same primer pair as before assuming that there would be less signal in the IgG control than in the one using the specific antibody. However, the results looked really strange - no signal at all in both samples. Does anybody have a good method to test the quality of the library prior to sequencing?
Da Bric
I'm new in the ChIP-seq world. I have a good antibody that I know works well in ChIP and I also know some good target regions that I can use to validate the ChIP. After performing and validating a ChIP I used the ChIP'd material to construct a library to use for Solexa sequencing. To test if the library construction went fine I tried to perform qPCR validation using the same primer pair as before assuming that there would be less signal in the IgG control than in the one using the specific antibody. However, the results looked really strange - no signal at all in both samples. Does anybody have a good method to test the quality of the library prior to sequencing?
Da Bric
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