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Old 09-26-2011, 02:49 PM   #1
SeqVicious
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Location: New Hampshire

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Default Current read1, index, and read2 primers for HiSeq2000

Hi all,

I'm currently being thrown into creating a library prep for some samples in our lab. We plan on multiplexing because and we want to throw in upwards of 6 samples. My lab has created library preps in the past but they used a different sequencing facility and older technology. We still have the left over reagents but I don't know if they will be compatible with HiSeq2000. Can someone tell me what the current read1, index, and read2 sequencing primers are? I have enough "barcodes" but I need to know what the sequence on either side of those "barcodes" are.
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Old 09-26-2011, 03:12 PM   #2
SeqVicious
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Also, I've been trying to find these on my own but I guess I just don't know enough about this stuff yet. What are the SE and PE primers?
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