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Old 02-07-2017, 07:23 AM   #1
dacotahm
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Location: ND, USA

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Default Cheap size fractionation for nanopore sequencing?

Hello,

I'm doing some 2D nanopore sequencing and I'm a bit disappointed with the number and cost of third-party products recommended in the protocols (specifically 2D Genomic DNA selecting for long reads). Does anyone know of a reasonably cheap product or method for fragmentation and size selecting 20-50kb or 100+kb gDNA fragments?

The protocol recommends Bluepippin which we don't have and the cartridges cost as much as the nanopore kit. I'd like to keep the prep and sequencing in-house.

Thanks for any advice!
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