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Old 03-16-2017, 04:37 AM   #1
tfh4
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Location: Arkansas

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Default Custom Yeast Genomic 70-mer Oligonucleotide Microarray chip NCBI GEO dataset?

How to extract the numerical values for plotting time series curves for this Custom Yeast Genomic 70-mer Oligonucleotide Microarray chip NCBI GEO dataset?
How to extract the numerical expression values for plotting time series curves for this Custom Yeast Genomic 70-mer Oligonucleotide Microarray NCBI GEO dataset?
I would like to plot the mRNA expression time series curves based on the data of the following NcBI GEO dataset.
Series GSE3705
The title of this dataset is:
JM43 cells grown aerobically in galactose and treated with Antimycin A
The URL of this dataset is:
https://www.ncbi.nlm.nih.gov/geo/que...gi?acc=GSE3705
The citation of the publication, which goes along with this dataset, is:
Lai LC, Kissinger MT, Burke PV, Kwast KE. Comparison of the transcriptomic "stress response" evoked by antimycin A and oxygen deprivation in Saccharomyces cerevisiae. BMC Genomics 2008 Dec 23;9:627. PMID: 19105839.
This publication is free available at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2637875/
My problem is that the platform is

GPL1535 Custom Yeast Genomic 70-mer Oligonucleotide Microarray
What do I need to do to extract the numerical gene expression values from this dataset, which are most meaningful for plotting time series curves?
Thanks a lot
Thomas
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