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Old 09-18-2012, 03:59 PM   #1
mwatling
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Location: Seattle

Join Date: Sep 2012
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Default Low 260/230 for MasterPure Gram+ Kit

I'm using Masterpure's Gram Positive DNA purification kit, trying to isolate
DNA from Propionibacterium acnes. Each attempt I get really graphs from the nanodrop.

I defnitely have DNA in my samples, because i'll have a high absorbance at 260, but sometimes the absorbance is even HIGHER at 230 nm.

I've tried doing an extra purification step, where I repeat the steps where you add isopropanol, spin at 4C, remove the isopropanol, wash with ethanol, and resuspend in 35ul TE.

Has anyone experienced DNA isolation where they had a high 230 absorbance, and what did you do to fix it?
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230, acnes, dna, isolation, masterpure

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