SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
Cutting corners with Illumina Sample Prep Rocketknight Sample Prep / Library Generation 21 03-29-2013 10:17 AM
Intra-sample variability, Illumina TruSeq mRNA eab General 20 07-16-2011 12:32 PM
Informatics cause of intra-sample variability, Illumina TruSeq mRNA eab Bioinformatics 0 07-13-2011 08:16 AM
Intra-sample variability, Illumina TruSeq mRNA eab General 0 07-12-2011 07:50 AM
uMACS cDNA kits for mRNA-seq sample prep ktabbada RNA Sequencing 0 10-16-2009 04:17 AM

Reply
 
Thread Tools
Old 01-08-2010, 03:31 AM   #1
ik76
Junior Member
 
Location: London, UK

Join Date: Jan 2010
Posts: 9
Default questions about Illumina mRNA sample prep

Hi,
We are currently using a standard Illumina mRNA protocol and have experienced two problems :

i) some genomic contamination
ii) inefficient depletion (using the Ribo- kit)

Has somebody experienced the same problems and has some words of advice?
Thank you in advance.
ik76 is offline   Reply With Quote
Old 01-12-2010, 10:50 AM   #2
Colinmt
Junior Member
 
Location: Utah

Join Date: Feb 2009
Posts: 2
Default

To remove genomic DNA contamination you might try TURBO-DNA free from Ambion before Ribominus treatment. This approach has worked well for us.
Unfortunately, the Ribominus kit does not remove all rRNAs so you'll still see some contamination, especially if you have a little degradation of your RNA
preparation.
Colinmt is offline   Reply With Quote
Old 01-19-2010, 03:38 AM   #3
runemoe
Junior Member
 
Location: Oslo, Norway

Join Date: Jun 2009
Posts: 8
Default

We have used the DNAse-TURBO kit with great success
http://www.ambion.com/catalog/CatNum.php?2238
runemoe is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 09:34 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2017, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO