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Old 06-14-2016, 04:29 AM   #1
Junior Member
Location: UK

Join Date: Jun 2016
Posts: 1
Default Tapestation mistake made

Hi all,

When I used Tapestation last time, I accidentally forgot to remove the caps of the tubes. There was error warning and after I cleaned up the stuck tips I re-ran some RNAs (only 4 out of 16 lanes in the tape can be detected). The run seemed work normally and I did get my results. The only thing I am worried is that apart from empty control and ladder, my two RNA samples (Arabidopsis roots) got RIN 10.0. While the RNA gel looked great, I still can't believe they got 10! The ladder looks fine and my RNA concentration is around 100ng/ul, lower than what I got from Qubit but comparable.
So I want to ask do you think my mistake (forgot to remove cap) can affect the performance of the machine? Did anyone have the same experience?
cambium2016 is offline   Reply With Quote
Old 03-06-2017, 01:07 PM   #2
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Location: Austin

Join Date: Mar 2017
Posts: 1

Hi,Did you figure that out what was the reason. Did you have to repair the Tapestation? We recently had a similar incidence and the tubes are stuck inside.TIA
gayatri281 is offline   Reply With Quote

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