SEQanswers

Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation



Similar Threads
Thread Thread Starter Forum Replies Last Post
ATAC-seq (Read1/Read2/Index Sequencing Primers?) emacalate Epigenetics 1 08-30-2016 11:11 AM
HELP!!!! How stable are Nextera Index primers? Phuray Illumina/Solexa 3 08-13-2015 11:36 PM
Annealing index and primers for multiplexing?? alisrpp Sample Prep / Library Generation 1 03-09-2011 07:51 PM
Annealing index and primers for multiplexing alisrpp Illumina/Solexa 0 02-22-2011 05:12 PM

Reply
 
Thread Tools
Old 01-30-2017, 01:17 PM   #1
kgoglin
Member
 
Location: San Diego

Join Date: Dec 2014
Posts: 12
Default PCR index primers for Illumina RNA-Seq

We currently use Illumina's ScriptSeq index primers for PCR amplifying cDNA produced by Wafergen's PrepX mRNA protocol. The ScriptSeq index primers only allow for 48 indexes. Are there any other primer sets with greater than 48 indexes that are compatible with Wafergen's PrepX protocol?
kgoglin is offline   Reply With Quote
Old 01-31-2017, 06:44 AM   #2
Olaf Blue
Member
 
Location: Madison, WI

Join Date: Nov 2010
Posts: 53
Default

The ScriptSeq user guide provides the complete sequences of the reverse/index primers. If a user so desires, they can order their own custom primers from any good oligo/primer manufacturer and simply use index sequences not provided. Note that ScriptSeq uses indexes 17, 24 and 26 which are not "standard" illumina indexes of the same number (ILMN reserved those for the future). These indexes were designed prior to the purchase of Epicentre by Illumina.
Olaf Blue is offline   Reply With Quote
Old 02-02-2017, 12:25 PM   #3
kgoglin
Member
 
Location: San Diego

Join Date: Dec 2014
Posts: 12
Default

Yeah. I guess we could just make our own.

Thanks!
kgoglin is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 02:23 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2017, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO