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Old 02-23-2017, 12:56 AM   #1
Misa
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Location: Sweden

Join Date: Aug 2016
Posts: 4
Default 2500bp band on gel after mRNA library build

Hi,
I'm having problems with an extra band on the gel following my library preparation (I've used Illumina TruSeq Stranded mRNA kit). At first I had two extra bands, one of smaller fragments and one larger, beside the smear from my library. The smaller one was cleared out following Illuminas instructions to do a second final cleanup. But the large band around 2500bp still remained.

The suggestion from Illumina was that it was beads that had tagged along so I was adviced to make another 5-10 min on the magnetic stand. However, the band is still there. Anyone that have had the same problem and have any suggestions how to deal with it?

The samples have been run on a RNA-tape on TapeStation, two samples, the last one in duplicate.
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