Next Generation Sequencing for Dummies

avierstr · 02-27-2017, 01:32 AM

Hi all,

I finally found some time to update my presentation from 2012. A lot changed in the NGS world in those years. I originally posted the link in http://seqanswers.com/forums/showthread.php?t=19067 but since the title does not cover the complete presentation, I've made a new post.

155 slides about the mostly used systems today, about applications (mainly for biology research) and some things to take into consideration and think about. The presentation and file with explanation can be downloaded from my webpage:
http://users.ugent.be/~avierstr/nextgen/nextgen.html

GenoMax · 02-27-2017, 03:32 AM

A very comprehensive effort. Thank you Andy!

This may be a good reference to go back to as technologies die out over time (e.g. 454, SOLiD).

rnaseq2017 · 03-23-2017, 09:45 AM

Excellent for beginers like me!

Brian Bushnell · 03-23-2017, 01:03 PM

Thanks! This is also very useful for non-beginners.

shchaoguo · 03-23-2017, 02:13 PM

Thank you and very informative.

avierstr · 03-27-2017, 01:48 AM

I have posted an updated version on March 27th 2017.

Updates about:
- Oxford Nanopore
- Some minor adjustments here and there

Added info about:
- SeqLL True Single Molecule Sequencing (Helicos technology)
- 2D transcriptomics

Available :
http://users.ugent.be/~avierstr/nextgen/nextgen.html

avierstr · 11-07-2017, 03:59 AM

I have posted an updated version on November 7th 2017.

Updates about:
- added a slide (nr. 110) how errors are introduced in sequences
- Some minor adjustments here and there

Available :
http://users.ugent.be/~avierstr/nextgen/nextgen.html

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
fastq-dump for dummies	UpsetNotMad Scientist	Bioinformatics	15	02-10-2014 01:56 PM
PubMed: Next-generation sequencing demands next-generation phenotyping.	Newsbot!	Literature Watch	0	07-31-2012 02:00 AM
PubMed: 'Next-generation' sequencing becomes 'now-generation'.	Newsbot!	Literature Watch	0	01-22-2012 02:23 AM
Analyzing NGS-data for dummies/biologists: suggestions for software?	MicroOmicsMan	Bioinformatics	6	12-22-2009 07:46 PM

02-27-2017, 01:32 AM	#1
avierstr Member Location: Ghent, Belgium Join Date: Apr 2012 Posts: 18	Next Generation Sequencing for Dummies Hi all, I finally found some time to update my presentation from 2012. A lot changed in the NGS world in those years. I originally posted the link in http://seqanswers.com/forums/showthread.php?t=19067 but since the title does not cover the complete presentation, I've made a new post. 155 slides about the mostly used systems today, about applications (mainly for biology research) and some things to take into consideration and think about. The presentation and file with explanation can be downloaded from my webpage: http://users.ugent.be/~avierstr/nextgen/nextgen.html

03-23-2017, 09:45 AM	#3
rnaseq2017 Junior Member Location: Germany Join Date: Mar 2017 Posts: 2	Thanks! Excellent for beginers like me!

02-27-2017, 03:32 AM	#2
GenoMax Senior Member Location: East Coast USA Join Date: Feb 2008 Posts: 6,697	A very comprehensive effort. Thank you Andy! This may be a good reference to go back to as technologies die out over time (e.g. 454, SOLiD).

03-23-2017, 01:03 PM	#4
Brian Bushnell Super Moderator Location: Walnut Creek, CA Join Date: Jan 2014 Posts: 2,706	Thanks! This is also very useful for non-beginners.

03-23-2017, 02:13 PM	#5
shchaoguo Junior Member Location: California, US Join Date: Aug 2014 Posts: 1	Thank you and very informative.

03-27-2017, 01:48 AM	#6
avierstr Member Location: Ghent, Belgium Join Date: Apr 2012 Posts: 18	I have posted an updated version on March 27th 2017. Updates about: - Oxford Nanopore - Some minor adjustments here and there Added info about: - SeqLL True Single Molecule Sequencing (Helicos technology) - 2D transcriptomics Available : http://users.ugent.be/~avierstr/nextgen/nextgen.html

11-07-2017, 03:59 AM	#7
avierstr Member Location: Ghent, Belgium Join Date: Apr 2012 Posts: 18	I have posted an updated version on November 7th 2017. Updates about: - added a slide (nr. 110) how errors are introduced in sequences - Some minor adjustments here and there Available : http://users.ugent.be/~avierstr/nextgen/nextgen.html