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Old 03-03-2017, 01:53 PM   #1
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Location: Columbus, Ohio

Join Date: Mar 2017
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Unhappy RNA Purification Beads during First and Second Strand Synthesis

I'm using the Tru-seq Stranded mRNA Sample Preparation kit and I made the mistake of not removing the RNA from the RNA Purification Beads before starting the First strand synthesis and then second strand synthesis. The only step I missed was removing the beads, I added everything else and ran the incubations required for the first and second strand synthesis. Is everything okay to continue or will I have to start over?
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