Hi, all-
I'm wondering if anyone has experience with loading just one strand of a ds duplex onto a flow cell. In this case, it would be the antisense (3'--5') strand, so that elongation would occur during the first round of amplification on the flow cell. Would this work and form productive clusters?
I'd be happy with comments about the logic of the idea, even if you haven't tried it before.
Thanks much!
Deb
I'm wondering if anyone has experience with loading just one strand of a ds duplex onto a flow cell. In this case, it would be the antisense (3'--5') strand, so that elongation would occur during the first round of amplification on the flow cell. Would this work and form productive clusters?
I'd be happy with comments about the logic of the idea, even if you haven't tried it before.
Thanks much!
Deb
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