While processing IsoSeq data, I observed that close to 10% of the films had adapter splitting problems.
The pattern is shown in the graphic hereunder with 3 examples :
Each graph correspond to one film (ZMW) which contains 20 ordered reads.
The graph shows the read length found after adapter splitting.
The one on the top is as awaited.
The second one shows a couple of reads which have not been split in position 13.
The last shows four errors.
When films contain multiple reads (let's say over 5, which is common in IsoSeq), it should be quiet easy to compare each read length to the median and decide if there has been a problem in the adapter splitting.
The pattern is shown in the graphic hereunder with 3 examples :
Each graph correspond to one film (ZMW) which contains 20 ordered reads.
The graph shows the read length found after adapter splitting.
The one on the top is as awaited.
The second one shows a couple of reads which have not been split in position 13.
The last shows four errors.
When films contain multiple reads (let's say over 5, which is common in IsoSeq), it should be quiet easy to compare each read length to the median and decide if there has been a problem in the adapter splitting.
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