When using the 5500 adapters and the Roche EZ Exome capture kit should I block the whole sequence of the P1 (41bp) and Standard (53bp) adapters during hybridisation? or can I just block the 3' end of each adapter...or just the Library PCR section?
I know Baylor used truncated adapters with their SOLiD 4 protocol and I could use this at the P1 end but they don't use the P2/barcode/IA.
I know Baylor used truncated adapters with their SOLiD 4 protocol and I could use this at the P1 end but they don't use the P2/barcode/IA.
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