Exploring the possibility of using this kit for our hyb-capture library prep front-end process. Does anyone know the specific enzymes that make up the cocktails? As well, is the enzymatic fragmentation random or does it target specific sequences? Is it known what the fragmentation enzymes are? Thanks
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My Qiagen rep was just here talking about this. He claimed that it isn't sequence specific cleaving at all but beyond that I couldn't get much info out of him. Showed some data comparing sequencing sites to covaris sheared DNA. They didn't have any company data on what I'd call high gc genomes so he's going send me a kit to try on some 75% GC streptococcusMicrobial ecologist, running a sequencing core. I have lots of strong opinions on how to survey communities, pretty sure some are even correct.
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Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
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03-22-2024, 06:39 AM -
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The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.
Avian Conservation
Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...-
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03-08-2024, 10:41 AM -
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