Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • RNAseq library for polysome profiling (monosome and polysome-associated RNA)

    Hi there,

    I am interested in performing polysome profiling with downstream application for RNAseq of monosome and polysome-associated RNAs.

    Has anyone had much experience with choice of library preparations for monosome and polysome-associated RNAs?

    ribosomal RNA surely is to be more abundant in these samples compared to total RNA extractions? This would lead me towards preferring ribosome-depletion (e.g. riboZero) as opposed to polyA. However PolyA is better for intact fragments and we also intend to sequence total RNA where polyA would be preferred. If we want to compare sequencing between total RNA and mono/poly RNAs it would make sense to use the same library prep?

    If you have any experience in choice of library prep (poly A vs ribo-depletion) for polysome-profiling RNAseq experiments please let me know which method you preferred and why

    Many thanks,

Latest Articles

Collapse

  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM
  • seqadmin
    Techniques and Challenges in Conservation Genomics
    by seqadmin



    The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

    Avian Conservation
    Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
    03-08-2024, 10:41 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Yesterday, 06:37 PM
0 responses
8 views
0 likes
Last Post seqadmin  
Started by seqadmin, Yesterday, 06:07 PM
0 responses
8 views
0 likes
Last Post seqadmin  
Started by seqadmin, 03-22-2024, 10:03 AM
0 responses
49 views
0 likes
Last Post seqadmin  
Started by seqadmin, 03-21-2024, 07:32 AM
0 responses
67 views
0 likes
Last Post seqadmin  
Working...
X