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  • What does TopHat do with discordantly mapping PE reads?

    What does TopHat do with discordantly mapping paired end reads -- either one read fails to align or the two cannot be aligned in a manner compatible with appropriate constraints (different chromosomes, wrong orientation on same chromosome, too far apart for intron size constraint)?

    Also, a feature request: it would be useful if TopHat dumped a FASTQ file of reads it couldn't align. But, that's lazy me talking -- I'm writing an unaligned puller right now.

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  • seqadmin
    Current Approaches to Protein Sequencing
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    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM
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    03-22-2024, 06:39 AM

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