Hi all,
I want to construct an RNA-Seq library used for paired-end sequencing using HiSeq2000 and need a protocol. I have found many version of modified protocol in PubMed, such as construction of strand-specified RNA-seq library. I also found some protocol of RNA-Seq library preparation kits, but I don't want to used these kit and the protocol is not detailed enough.
Is there anyone have such protocol or know where I can get it?
Jian-You
I want to construct an RNA-Seq library used for paired-end sequencing using HiSeq2000 and need a protocol. I have found many version of modified protocol in PubMed, such as construction of strand-specified RNA-seq library. I also found some protocol of RNA-Seq library preparation kits, but I don't want to used these kit and the protocol is not detailed enough.
Is there anyone have such protocol or know where I can get it?
Jian-You
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