Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • genome STRiP Error

    Hello all,

    I am getting the following error when trying to run the SVPreprocess of Genome STRiP. Any ideas of what is wrong would be great.
    INFO 18:36:19,129 QScriptManager - Compiling 2 QScripts
    INFO 18:36:32,249 QScriptManager - Compilation complete
    INFO 18:36:36,870 HelpFormatter - ---------------------------------------------------------
    INFO 18:36:36,871 HelpFormatter - Program Name: org.broadinstitute.sting.queue.QCommandLine
    INFO 18:36:36,871 HelpFormatter - Program Args: -S /shared/local/svtoolkit/qscript/SVPreprocess.q -S /shared/local/svtoolkit/qscript/SVQScript.q -gatk /shared/local/svtoolkit/lib/gatk/GenomeAnalysisTK.jar -cp /shared/local/svtoolkit/lib/SVToolkit.jar:/shared/local/svtoolkit/lib/gatk/GenomeAnalysisTK.jar -configFile /shared/local/svtoolkit/conf/conf/genstrip_parameters.txt -tempDir /storage/isu/reecy_lab/tmp_eric/STRiP_temp -md /storage/isu/reecy_lab/tmp_eric/STRiP_metadata -R /shared/local/svtoolkit/installtest/data/human_b36_chr1.fasta -genomeMaskFile /shared/local/svtoolkit/installtest/data/human_b36_chr1.mask.fasta -genderMapFile /shared/local/svtoolkit/installtest/data/installtest_gender.map -I /shared/local/svtoolkit/installtest/data/installtest.bam -run
    INFO 18:36:36,871 HelpFormatter - Date/Time: 2011/03/27 18:36:36
    INFO 18:36:36,871 HelpFormatter - ---------------------------------------------------------
    INFO 18:36:36,872 HelpFormatter - ---------------------------------------------------------
    INFO 18:36:36,874 QCommandLine - Scripting SVPreprocess
    INFO 18:36:37,034 QCommandLine - Added 7 functions
    INFO 18:36:37,034 QGraph - Generating graph.
    INFO 18:36:37,086 QGraph - Running jobs.
    INFO 18:36:37,141 ShellJobRunner - Starting: java -Xmx4g -Djava.io.tmpdir=/storage/isu/reecy_lab/tmp_eric/STRiP_temp -cp /shared/local/svtoolkit/lib/SVToolkit.jar:/shared/local/svtoolkit/lib/gatk/GenomeAnalysisTK.jar org.broadinstitute.sting.gatk.CommandLineGATK -T ComputeInsertSizeDistributions -R /shared/local/svtoolkit/installtest/data/human_b36_chr1.fasta -I /shared/local/svtoolkit/installtest/data/installtest.bam -O /storage/isu/reecy_lab/tmp_eric/STRiP_metadata/isd/installtest.hist.bin -md /storage/isu/reecy_lab/tmp_eric/STRiP_metadata -createEmpty
    INFO 18:36:37,142 ShellJobRunner - Output written to /home/users/ercfrtz/[email protected]
    ERROR 18:36:37,189 ShellJobRunner - Error: java -Xmx4g -Djava.io.tmpdir=/storage/isu/reecy_lab/tmp_eric/STRiP_temp -cp /shared/local/svtoolkit/lib/SVToolkit.jar:/shared/local/svtoolkit/lib/gatk/GenomeAnalysisTK.jar org.broadinstitute.sting.gatk.CommandLineGATK -T ComputeInsertSizeDistributions -R /shared/local/svtoolkit/installtest/data/human_b36_chr1.fasta -I /shared/local/svtoolkit/installtest/data/installtest.bam -O /storage/isu/reecy_lab/tmp_eric/STRiP_metadata/isd/installtest.hist.bin -md /storage/isu/reecy_lab/tmp_eric/STRiP_metadata -createEmpty
    ERROR 18:36:37,190 ShellJobRunner - /storage/isu/reecy_lab/tmp_eric/STRiP_temp/.exec2786000665283577153: line 2: java: command not found

    INFO 18:36:37,195 ShellJobRunner - Starting: java -Xmx4g -Djava.io.tmpdir=/storage/isu/reecy_lab/tmp_eric/STRiP_temp -cp /shared/local/svtoolkit/lib/SVToolkit.jar:/shared/local/svtoolkit/lib/gatk/GenomeAnalysisTK.jar org.broadinstitute.sting.gatk.CommandLineGATK -T ComputeReadDepthCoverage -R /shared/local/svtoolkit/installtest/data/human_b36_chr1.fasta -I /shared/local/svtoolkit/installtest/data/installtest.bam -O /storage/isu/reecy_lab/tmp_eric/STRiP_metadata/depth/installtest.depth.txt -md /storage/isu/reecy_lab/tmp_eric/STRiP_metadata -genomeMaskFile /shared/local/svtoolkit/installtest/data/human_b36_chr1.mask.fasta -minMapQ 10
    INFO 18:36:37,195 ShellJobRunner - Output written to /home/users/ercfrtz/[email protected]
    ERROR 18:36:37,217 ShellJobRunner - Error: java -Xmx4g -Djava.io.tmpdir=/storage/isu/reecy_lab/tmp_eric/STRiP_temp -cp /shared/local/svtoolkit/lib/SVToolkit.jar:/shared/local/svtoolkit/lib/gatk/GenomeAnalysisTK.jar org.broadinstitute.sting.gatk.CommandLineGATK -T ComputeReadDepthCoverage -R /shared/local/svtoolkit/installtest/data/human_b36_chr1.fasta -I /shared/local/svtoolkit/installtest/data/installtest.bam -O /storage/isu/reecy_lab/tmp_eric/STRiP_metadata/depth/installtest.depth.txt -md /storage/isu/reecy_lab/tmp_eric/STRiP_metadata -genomeMaskFile /shared/local/svtoolkit/installtest/data/human_b36_chr1.mask.fasta -minMapQ 10
    ERROR 18:36:37,218 ShellJobRunner - /storage/isu/reecy_lab/tmp_eric/STRiP_temp/.exec8096455693624243640: line 2: java: command not found

    INFO 18:36:37,220 QCommandLine - Done with errors
    INFO 18:36:37,222 QGraph - -------
    INFO 18:36:37,229 QGraph - Failed: java -Xmx4g -Djava.io.tmpdir=/storage/isu/reecy_lab/tmp_eric/STRiP_temp -cp /shared/local/svtoolkit/lib/SVToolkit.jar:/shared/local/svtoolkit/lib/gatk/GenomeAnalysisTK.jar org.broadinstitute.sting.gatk.CommandLineGATK -T ComputeInsertSizeDistributions -R /shared/local/svtoolkit/installtest/data/human_b36_chr1.fasta -I /shared/local/svtoolkit/installtest/data/installtest.bam -O /storage/isu/reecy_lab/tmp_eric/STRiP_metadata/isd/installtest.hist.bin -md /storage/isu/reecy_lab/tmp_eric/STRiP_metadata -createEmpty
    INFO 18:36:37,230 QGraph - Log: /home/users/ercfrtz/[email protected]
    INFO 18:36:37,230 QGraph - -------
    INFO 18:36:37,231 QGraph - Failed: java -Xmx4g -Djava.io.tmpdir=/storage/isu/reecy_lab/tmp_eric/STRiP_temp -cp /shared/local/svtoolkit/lib/SVToolkit.jar:/shared/local/svtoolkit/lib/gatk/GenomeAnalysisTK.jar org.broadinstitute.sting.gatk.CommandLineGATK -T ComputeReadDepthCoverage -R /shared/local/svtoolkit/installtest/data/human_b36_chr1.fasta -I /shared/local/svtoolkit/installtest/data/installtest.bam -O /storage/isu/reecy_lab/tmp_eric/STRiP_metadata/depth/installtest.depth.txt -md /storage/isu/reecy_lab/tmp_eric/STRiP_metadata -genomeMaskFile /shared/local/svtoolkit/installtest/data/human_b36_chr1.mask.fasta -minMapQ 10
    INFO 18:36:37,231 QGraph - Log: /home/users/ercfrtz/[email protected]

Latest Articles

Collapse

  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin




    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
    04-22-2024, 07:01 AM
  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
59 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
57 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 09:21 AM
0 responses
51 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-04-2024, 09:00 AM
0 responses
56 views
0 likes
Last Post seqadmin  
Working...
X