Hi all,
I'm trying to generate a list of oligonucleotide primers for Sanger sequencing, such that for an arbitrarily large DNA sequence I would get an optimised oligonucleotide every ca. 500 bases. How is this best possible? Solutions I have thought along are:
- BatchPrimer 3 (http://probes.pw.usda.gov/batchprimer3/)
- Querying NCBI Primer-Blast through R => but is there a tool for that..?
Thanks!
Simon
I'm trying to generate a list of oligonucleotide primers for Sanger sequencing, such that for an arbitrarily large DNA sequence I would get an optimised oligonucleotide every ca. 500 bases. How is this best possible? Solutions I have thought along are:
- BatchPrimer 3 (http://probes.pw.usda.gov/batchprimer3/)
- Querying NCBI Primer-Blast through R => but is there a tool for that..?
Thanks!
Simon