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  • Barcoding for cfDNA?

    Hello,

    We will use barcoding system for cfDNA WES (both cancer and normal).
    In addition to cfDNA WES, we will perform tissue WES (same patients).
    My question is, if we do barcoding system for cfDNA WES, should we use barcoding system for tissue WES?

    Thank you in advance.

  • #2
    Barcoding in this context is vague. I wonder if you mean using indexed adapters for multiplex sequencing.

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    • #3
      Originally posted by nucacidhunter View Post
      Barcoding in this context is vague. I wonder if you mean using indexed adapters for multiplex sequencing.
      Yes. Barcoding here means indexing.

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      • #4
        Using indexes would give more options for multiplex sequencing with no adverse effect.

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